Extraction of Quercetin from Citrus Sinensis using Ultrasound Assisted Hydrotropic Extraction
DOI:
https://doi.org/10.56147/jbhs.2.3.35Keywords:
- Quercetin,
- Hydrotropic extraction,
- Ultrasound-assisted extraction,
- Sodium benzoate,
- Orange peels,
- Response surface methodology
Abstract
Background: Quercetin, a bioactive flavonoid with significant antioxidant and anti-inflammatory properties, suffers from poor aqueous solubility, limiting its bioavailability.
Objective: This study aimed to enhance quercetin solubility and extraction efficiency from orange peels using Ultrasound-Assisted Hydrotropic Extraction (UAHE) with sodium benzoate.
Methods: The molar absorptivity of quercetin was determined via UV spectroscopy. Hydrotropic solubilization was performed at varying sodium benzoate concentrations (0-3 mol/L). Process parameters (hydrotrope concentration, extraction time, solid loading) were optimized using Response Surface Methodology (RSM).
Results: Maximum solubility (22.8 × 10⁻³ mol/L) was achieved at 2.6 mol/L hydrotrope concentration. Optimal UAHE conditions (3 mol/L, 30 min, 20% w/v) yielded 26.2 µg/g quercetin, with sodium benzoate outperforming sodium benzene sulphonate. RSM confirmed hydrotrope concentration as the most influential factor (p<0.05).
Conclusion: UAHE with sodium benzoate is a green, efficient method for quercetin extraction, offering potential for pharmaceutical applications.
